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1、摘要刺玫果學(xué)名野薔薇,又名野刺玫果,山刺玫果,系薔薇科薔薇屬植物山刺玫的成熟果實(shí)。廣泛分布于我國的東北地區(qū)，尤以黑龍江省貯量最為豐富?，F(xiàn)代藥理學(xué)研究表明：刺玫果含有豐富的營養(yǎng)成分及生物活性物質(zhì)，具有健脾理氣、養(yǎng)血調(diào)經(jīng)、抗衰老、防治心血管疾病、增強(qiáng)免疫及促智等功效。刺玫果中所含的總黃酮類物質(zhì)具有預(yù)防和治療高血脂、血栓所致的心腦血管疾病的功效，且具有高效、低毒副作用的優(yōu)勢(shì)。因此純化刺玫果中的總黃酮就具有現(xiàn)實(shí)的科研意義。目前，常見的總黃酮純化方法有水提醇沉法（水醇法）、醇提水沉法（醇水法）、酸堿法、鹽析法、離子交換法和結(jié)晶法等。實(shí)踐表明，這些方法也存在
2、一定的局限性。近年出現(xiàn)了一些分離和純化的新方法，如絮凝沉淀法，大孔樹脂吸附法、超濾法、高速離心法等。本論文采用大孔樹脂法純化刺玫果提取物中的總黃酮。探索洗脫劑、pH值、上樣濃度等因素對(duì)純化過程的影響。采用紫外分光光度計(jì)法對(duì)總黃酮含量進(jìn)行了分析，最終確定了洗脫劑的濃度75%、上樣PH為5，上樣濃度為原液濃度，吸附速率為2Bv/h，洗脫速率為1Bv/h。該純化方法簡單，快捷，純化效率高，進(jìn)一步拓展了刺玫果的應(yīng)用領(lǐng)域和范圍，為刺玫果的全面和綜合利用提供科學(xué)的理論依據(jù)。關(guān)鍵詞：刺玫果總黃酮大孔樹脂法純化AbstractRosamultifloraisis
3、thescientificnameofRosadavuricapall，alsoknownasWildrosafruit.Rosamountainfruit,thisfruitistheRosamountainfruitofRosaceaegenusRosa.Itisgrowingineast-northChina,especiallyinprovinceofHeilongjiang.ModernpharmaceuticalresearchshowsthatRosadavuricaPallcontainsrichnutrientsandbioac
4、tivesubstances,anti-aging,preventionandtreatmentofcardiovasculardisease,enhancesimmunityandtheefficacyofnootropic,etc.TotalflavonoidsintheRosadavuricaPallpossessthefunctionofpreventionandtreatmentofhighbloodlipids,thrombosisduedtocardiovascularandcerebrovasculardiseases.Ithas
5、propertieslikehigh-efficiencyandtheadvantagesoflowtoxicitysideeffects.Thusitisworthindoingresearchonrefiningtotalflavonoidsinthefruit.Recently,therearetheseveralcommonmethodsinpurifyingFlavonoidssuchaswater-extractingalcohol-precipitating,alcohol-extractingwater-precipitating
6、,acid-basemethod,saltdopositing,io-exchangeandcrystallization,andsoon.Inpractice,thereisthelimitinallthesemethodreported.Somenewmethodsareinvestigatedlikeflocculationprecipitation,macroporousresinabsorption,ultrafiltration,high-speedcentrifugea-tion,andsoon. Inthisthesis,were
7、portextractionoftotalflavonoidsfromRosadavuricapallbyusingthemethodofMacroporousresin.ItwasinvestigatedbasingonhowthetypeofEluant,PHofsolution,concentrationofsamplesinfluencespurification.UsingUltravioletspectrophotometermethodtoanalyzeFlavonoids,Thismethodistoknowthecontento
8、ftotalFlavonoidsbymeasuringUltravioletabsorption.Asaresult,itwasfoun
