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《sirna沉默卵巢癌cxcr4基因?qū)β殉舶└骨晦D(zhuǎn)移的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、可北醫(yī)藥2013年12月第35卷第24期HebeiMedicalJournal，2013，Vol35DecNo．243701doi：10．3969／j．issn．1002—7386．2013．24．007·論著·siRNA沉默卵巢癌CXCR4基因?qū)β殉舶└骨晦D(zhuǎn)移的影響燕霞燕鋒張靜【摘要】目的研究應(yīng)用基因靶向治療對(duì)卵巢癌腹腔轉(zhuǎn)移的實(shí)驗(yàn)療效觀察。方法制作好的卵巢癌大鼠模型60只，采用隨機(jī)數(shù)字表法隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組，實(shí)驗(yàn)組應(yīng)用基因靶向治療，對(duì)照組采用陰性治療，siRNA沉默卵巢癌CXCR4基因后，觀察CXCR4蛋白的表達(dá)情況，對(duì)卵巢
2、癌細(xì)胞增殖的影響，觀察應(yīng)用基因靶向治療后腹腔轉(zhuǎn)移情況，評(píng)價(jià)大鼠生存情況。結(jié)果實(shí)驗(yàn)組在治療后腹水中的CXCR4顯著減少(P<0．05)；對(duì)照組治療后腹水中的CXCR4量顯著性增加(P<0．05)；實(shí)驗(yàn)組與對(duì)照組相比，治療前腹水中CXCR4，差異無(wú)統(tǒng)計(jì)學(xué)意義(P>0．05)；治療后差異有統(tǒng)計(jì)學(xué)意義(P<0．05)。實(shí)驗(yàn)組腹水抽出量與實(shí)驗(yàn)組相比，明顯減少(P<0．05)。結(jié)論應(yīng)用基因靶向治療方法siRNA沉默卵巢癌CXCR4基因?qū)β殉舶└骨晦D(zhuǎn)移，具有較好的療效，臨床預(yù)后較好?！娟P(guān)鍵詞】卵巢癌；腹腔轉(zhuǎn)移；基因靶向治療【中圖分類號(hào)】R737
3、．31【文獻(xiàn)標(biāo)識(shí)碼】A【文章編號(hào)】1002—7386(2013)24—3701—03EfectofsmallinterferingRNA—inducedCXCR4silencegeneonpe~toncalmetastasisofova~ancancerinratsYANX／a，YANFeng．zHANGJing．MedicalDepartment，AffiliatedHospitalofHebeiEngineeringUnivemity，Hebei，Handan056002，China【Abstract】0bjectiveToo
4、bservethetherapeuticeffectofCXCchemokinereceptor-4(cxcR4)．targetedtherapyonovariancancer．MethodsDMBAcottonthreadwasembeddedintotheOVariesofWisterratstoinduceovariancancermode1．Then60ratmodelswithovariancancerwererandomlydividedintoexperimentalgroupandcontrolgroupbymea
5、nsofrandomdigitstable．Theratsinexperimentalgroupweretreatedbygenetargetedtherapy，however，theratsincontrolgroupweretreatedbynegativetherapy．AftersiRNAsilencingovariancancerCXCR4gene，theexpressionofCXCR4protein，itseffectoncellproliferationofovariancancer，peritonealcavit
6、ymetastasisaftergenetargetedtherapywereobserved，andthesurvivalconditionofratsWasevaluated．ResultsAftertreatmenttheCXCR4levelsinascitesofexperimentalgroupweresignificantlydecreased(P<0．05)，however，theCXCR4levelsinascitesofcontrolgroupweresignificantlyincreased(P<0．05)．
7、TherewerenosignificantdifferencesintheCXCR4levelsinascitesbeforetreatmentbetweenexperimentalgroupandcontrolgroup(P>0．05)，however，thereweresignificantdifferencesintheCXCR4levelsinascitesaftertreatmentbetweenexperimentalgroupandcontrolgroup(P<0．05)．Ascomparedwiththatinc
8、ontrolgroup。theascitesextractionvolumeinexperimentalgroupwasobviouslydecreased(P<0．05)．ConclusionApplicationofgenetargetedth
