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1、才直物保2014.40(2):48—52PlantProtection綠豆立枯絲核菌研究初報(bào)王彥,曹志敏,張志肖,蘇秋竹,范保杰,劉長(zhǎng)友,田靜(河北省農(nóng)林科學(xué)院糧油作物研究所,河北省作物遺傳育種重點(diǎn)實(shí)驗(yàn)室,石家莊050035)摘要本研究通過形態(tài)學(xué)、菌絲融合群和致病力測(cè)定研究,對(duì)從河北省石家莊地區(qū)綠豆種植區(qū)分離的9O個(gè)立枯絲核菌進(jìn)行鑒定。在9O個(gè)分離物中有71個(gè)屬于AG4,占供試分離物的78.89,2個(gè)屬于AG2—2,占供試分離物的2.22,另外17個(gè)分離物與標(biāo)準(zhǔn)菌株不融合,占供試分離物的18.89;屬
2、于AG4的71個(gè)分離物中,55個(gè)與AG4完全融合(占77.46),16個(gè)與ACA不完全融合(占22.54)。在溫室條件下采用人工接菌法對(duì)4O個(gè)代表性分離物的致病力進(jìn)行測(cè)定,發(fā)現(xiàn)不同分離物對(duì)同一品種的致病力存在差異,其中分離物R3、R6、R9、R35致病力最強(qiáng),分離物R23、R31—1致病力最弱。屬于AG4的分離物R3、R6、R9、R35與其他供試分離物致病力差異極顯著;屬于未知群體的分離物R20、R29和R24之間致病力差異極顯著;屬于AG2—2的分離物1、R31—1致病力較弱,且差異不顯著。關(guān)鍵詞綠
3、豆;立枯絲核菌;菌絲融合群;致病力中圖分類號(hào):S435.22文獻(xiàn)標(biāo)識(shí)碼:ADOI:10.3969/j.issn.0529—1542.2014.02.009PreliminaryidentificationofRhizoctoniasolanicausingmungbeanseedlingblightWangYan,CaoZhimin,ZhangZhixiao,SuQiuzhu,F(xiàn)anBaojie,LiuChangyou,TianJing(InstituteofCerealandOilCrops,Heb
4、eiAcademyofAgriculturalandForestrySciences/LaboratoryofCropGeneticandBreedingofHebeiProvince,Shijiazhuang050035,China)AbstractNinetyisolatesofRhizoctoniasolani,collectedfromthemungbeanfieldinShijiazhuangregionwerecharacterizedbymorphology,anastomosisgro
5、upandpathogenicitytest.Theresultsshowedthatallisolatesweremultinucleate.Seventy-oneisolatesbelongedtotheAG4,accountingfor78.89%ofthetestedisolates,55isolateswerecompletelyfusedwithAG4(77.46%),16strainswereincompletelyfusedwithAG4(22.54%).Twoiso—fatesbel
6、ongedtotheAG2—2。accountingfor2.22%Therewere17strainsthatcouldnotfusewithanystandardstrains,accountingfor18.89%.VirulencetestingreenhousedemonstratedthattheisolatesR3,R6,R9andR35werehighlyvirulent,butisolatesR23andR31-1wereweaklyvirulent.Therewereextreme
7、lysignificantdiffer—encebetweentheisolatesR3,R6,R9,R35andtheothersbelongingtoAG·4,alsobetweentheisolatesR20,R29andR24belongingtounknowngroup.TheisolatesR21andR31—1belongingtoAG2·2wereweaklyvirulentandhadnosignificantdifferenceinvirulence.Keywordsmungbea
8、n,Rhizoctoniasolani;anastomosisgroup;pathogenicity綠豆是豇豆屬中的一個(gè)栽培種,具有較高的營(yíng)有分布,且寄主范圍極廣。立枯絲核菌屬于多核絲養(yǎng)價(jià)值和藥用價(jià)值[】],近年來,國內(nèi)外需求量逐年上核菌,主要引起植物的爛種、根腐、苗期猝倒和立枯升,綠豆種植面積也逐年擴(kuò)大l2]。由于綠豆的連年病,導(dǎo)致作物缺苗斷垅,被認(rèn)為是最具破壞力的土傳種植,綠豆病害發(fā)生也隨之加重,導(dǎo)致綠豆產(chǎn)量和質(zhì)植物病原物之一L3]。本文從形態(tài)鑒定、