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1、生態(tài)學(xué)雜志ChineseJournalofEcology2014，33(2)：394-399一株拮抗立枯絲核菌的放線菌篩選、鑒定及生理特性術(shù)張靖宜張倩茹～項文化魏樹和牟文燕(中南林業(yè)科技大學(xué)生命科學(xué)與技術(shù)學(xué)院，長沙410004；中國科學(xué)院沈陽應(yīng)用生態(tài)研究所污染生態(tài)與環(huán)境工程重點(diǎn)實驗室，沈陽110016)摘要通過對峙實驗，從采集自黃渤海海域的雙齒圍沙蠶(Perinereisaibuhitensis)中分離到一株內(nèi)生放線菌SCF一18，并對其抑菌活性進(jìn)行分析。SCF．18對立枯絲核菌(Rhizoctoniasolani)和小麥根腐
2、病菌(Bipolarissorokiniana)都具有拮抗作用，且對立枯絲核菌生長的拮抗效果最為明顯，生長抑制率為89．78％。抑菌試驗結(jié)果表明：菌株SCF．18發(fā)酵濾液可以抑制立枯絲核菌絲的生長，發(fā)酵液濃度越高，抑制力越強(qiáng)；當(dāng)發(fā)酵液濃度達(dá)到50％時，對兩種病原菌的抑制率分別為89．78％和80．26％。菌株SCF．18生長特性試驗結(jié)果表明：菌株SCF-18生長最適溫度為30℃，最適pH值為7；放線菌SCF一18耐鹽度可達(dá)5％。根據(jù)形態(tài)特征、培養(yǎng)特征、生理生化特性和16SrDNA序列分析，將放線菌SCF一18初步鑒定為淡紫灰鏈
3、霉菌(Streptomyceslavendulae)。本研究發(fā)現(xiàn)，SCF一18菌株是防治立枯絲核菌等病原菌的潛在優(yōu)良生防菌株，具有潛在的開發(fā)應(yīng)用價值。關(guān)鍵詞立枯絲核菌；雙齒圍沙蠶；淡紫灰鏈霉菌；16SrDNA；系統(tǒng)發(fā)育樹中圖分類號X171．5文獻(xiàn)標(biāo)識碼A文章編號1000—4890(2014)2—0394—06Screening，identificationandphysiologicalcharacteristicsofanactinomycetestrainagainstRhizoctoniasolani．ZHANGJing
4、—yi’，ZHANGQian．1"ll，XIANGWen—hua．WEIShu．he，MUWen—yan(Faculty0廠LireScienceandTechnology．CentralSouthUniversityofForestryandTechnology，Changsha410004，China；KeyLaboratoryo一．fPollutionEcologyandEnvironmentalEngineering，InstituteofAppliedEcology，ChineseAcademyofSciences，
5、Shenyang110016，China)．ChineseJournalofEcology，2014，33(2)：394-399．Abstract：Byusingdualculturetechnique，astrainofendophyticactinomycetenamedSCF一18wasscreenedoutandisolatedfromPerinereisaibuhitensiscollectedfromtheHuanghaiandBohaiSea，andthebio—activitiesoftheactinomyce
6、teagainstpathogenicfungiweredetermined．ThestrainSCF一18showedsignificantlyantagonisticactivitiestobothRhizoctoniasolaniandBipolarissorokiniana，particularlymorepotenttoR．solaniwiththemaximuminhibitionrateof89．78％．ThefermentationfiltrateofSCF．18couldinhibitthegrowthofR
7、．solaniinadose．dependedman—ner．Furthermore．thefermentationfiltrateofSCF．18in50％concentrationshowedthemaximumgrowthinhibitoryeffectstoR．solaniandH．sativumwithinhibitionratesof89．78％and80．26％．respectively．TheoptimumgrowthconditionforSCF．18wasat30℃withpH7．Thehighesttol
8、eranceofsalinityofSCF．18was5％．Basedontheanalysisofmorphology．culturalcharacteristics，physiologicalandbiochemicalproperties．a(chǎn)nd16SrDNAseque
