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1、HCN2陽性細胞在小鼠消化管肌間神經(jīng)叢內的分布熊承杰，梅峰，韓鵑，田衍平，江忠勇，周德山（第三軍醫(yī)大學組織學與胚胎學教研室，重慶400038）摘提要：目的探討（問題1：中文全稱?）(HCN2Asubtypeofchannelsofhyperpolarization-activatedcyclicnucleotide-gatedfamily,HCN2)HCN2刪除陽性細胞在小鼠胃腸道肌間神經(jīng)叢的分布。方法成年昆明小鼠的胃腸道，包括胃、小腸、結腸，制作全層鋪片免疫細胞化學染色HCN2陽性細胞和膽堿能神經(jīng)及cajal間質細胞(ICCs；interstitialCajalcells,ICCs)。結果成
2、年小鼠胃腸道肌間神經(jīng)叢的HCN2陽性細胞成簇緊密排列，多數(shù)陽性細胞為DogielIΙ型神經(jīng)元，陽性產(chǎn)物分別在細胞質胞漿和細胞膜，每個神經(jīng)節(jié)內可見數(shù)十個至數(shù)百個陽性細胞；神經(jīng)突起雖可見HCN2陽性產(chǎn)物，但主要存在于較粗的突起。HCN2陽性神經(jīng)元分布密度在胃腸道各段差異較大：結腸密度最高，胃次之，小腸比胃低，另外，；胃腸道的各截斷表達量也不一樣。利用膽堿能神經(jīng)元標志物(ChAT,膽堿乙酰轉移酶)和ICCs的標志物（c-kit蛋白）與HCN2進行雙重免疫染色可見HCN2與膽堿能神經(jīng)元共存，而未見HCN2與ICCs共存。結論HCN2離子通道廣泛表達于肌間神經(jīng)叢，存在于膽堿能神經(jīng)元，可能參與調控胃腸神經(jīng)
3、的興奮性，神經(jīng)遞質的釋放以及胃腸道神經(jīng)網(wǎng)絡的建立。關鍵詞：HCN2；肌間神經(jīng)叢；膽堿能神經(jīng)；cajal間質細胞；c-kit中圖法分類號：R322.8；R329.1ThedistributionofimmunoreactivecellsforHCN2inthemyentericnerveplexusofdigestivetractofmiceAbstract:Objectivetoinvestigatethedistributionofimmunoreactive(IR)cellsforHCN2,whichisonesubtypeofchannelsofhyperpolarization-act
4、ivatedcyclicnucleotide-gatedfamily,inthemyentericnerveplexusofgastrointestinaltractofmice.Methods:gastrointestinaltractsamples,whichincludestomach,smallintestineandcolon,frommatureKMmicewerecollectedforthepreparationofwhole-mountpreparationsexaminedbyimmunocytochemistryforIRcellsforHCN2andCholinergi
5、cnervesandICCs.ResultsMostofIRcellsforHCN2withinthemyentericplexusofmaturemicewereDogielIΙtype-neuronsandIRproductsoccurredonthecellmembranesandcytoplasm.theyoftengatheredinclusterintheganglia,whichcontainsdozenstohundredsofIRcellsforHCN2.IRproductscanalsobeseenonnervousprocess,butmajorityoccurredon
6、thosewhichwerethicker.ThedensityofdistributionforIRcellsforHCN2inthegastrointestinaltractisdifferent::colonishighest,stomachislowerthancolon,smallintestineislowest,andquantityofexpressionofeachcutofgastrointestinaltractisalsodifferent.ThemarkersofCholinergicnerves(ChAT)andICCs(c-kit)wereusedtodouble
7、-immunostainingwithHCN2,collocationofHCN2positivenervesandCholinergicnervescanbefound,butnocollocationofHCN2positivenervesandICCscanbefound.conclusionHCN2channelsarewidelydistributedinthemyentericnerv
