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1、HCN2陽性細胞在小鼠胃腸道肌間神經(jīng)叢內(nèi)的分布熊承杰，梅峰，韓鵑，田衍平，江忠勇，周德山（第三軍醫(yī)大學基礎醫(yī)學部組織學與胚胎學教研室，重慶市神經(jīng)科學研究所，重慶400038）【摘要】目的探討超級化激活環(huán)核苷酸調(diào)控的陽離子通道（hyperpolarization-activatedcyclicnucleotide-gatedchannel，HCN2）陽性細胞在小鼠胃腸道肌間神經(jīng)叢的分布。方法成年昆明小鼠的胃腸道，包括胃、小腸、結(jié)腸，制作全層鋪片，免疫細胞化學染色HCN2陽性細胞、膽堿能神經(jīng)及Caial間質(zhì)細胞(InterstitialCellsofC
2、ajal,ICCs)。結(jié)果成年小鼠胃腸道肌間神經(jīng)叢的HCN2陽性細胞成簇緊密排列，多數(shù)陽性細胞為DogielⅡ型神經(jīng)元，陽性產(chǎn)物分布在細胞質(zhì)和細胞膜，每個神經(jīng)節(jié)內(nèi)可見數(shù)個至數(shù)十個陽性細胞；神經(jīng)突起雖可見HCN2陽性產(chǎn)物，但主要存在于較粗的突起。HCN2陽性神經(jīng)元分布密度在胃腸道各段存在較大差異（P<0.05）：以結(jié)腸密度最高，胃次之，小腸略低。利用膽堿能神經(jīng)元標志物膽堿乙酰轉(zhuǎn)移酶（ChAT）和ICCs的標志物（c-kit蛋白）分別與HCN2通道進行雙重免疫染色，可見HCN2存在于膽堿能神經(jīng)元，未見HCN2存在于ICCs的胞體，可見ICCs的突起有H
3、CN2表達。結(jié)論HCN2離子通道廣泛表達于肌間神經(jīng)叢，主要表達于膽堿能神經(jīng)元，可能參與該神經(jīng)元調(diào)控胃腸神經(jīng)活動、神經(jīng)遞質(zhì)的釋放以及胃腸功能的調(diào)節(jié)?！娟P(guān)鍵詞】HCN2；腸肌叢肌間神經(jīng)叢；膽堿能神經(jīng)；Cajal間質(zhì)細胞；c-kit【中圖法分類號】R322.8；R329.1【文獻標志碼】AThedDistributionofHCN2-immunoreactivecellsforHCN2inthemyentericnerveplexusofmousedigestivetractofmiceXiongChengjie，,MeiFeng,，HanJuan，,T
4、ianYanping，,JiangZhongyong，,ZhouDeshan(DepartmentofHistologyandEmbryology，,CollegeofBasicMedicalSciencesMedicine，,ThirdMilitaryMedicalUniversity，,Chongqing,400038，,China))【Abstract】ObjectivetoToinvestigatethedistributionofhyperpolarization-activatedcyclicnucleotide-gatedchanne
5、l2-positivecells(HCN2)immunoreactive(IR)cellsforhyperpolarization-activatedcyclicnucleotide-gatedchannel(HCN2),inthemyentericnerveplexusofgastrointestinaltractofmice.Methods:gGastrointestinaltractsamples,whichincludedthestomach,smallintestineandcolon,frommatureKuMmingmicewerec
6、ollectedforthepreparationofwhole-mountpreparations,andthenexaminedbyimmunocytochemistryforHCN2,IRcellsforHCN2andCcholinergicnervesandinterstitialcellsofCajal(ICCs)ICCswithfluorescencemicroscopyandlaserconfocalmicroscopy.Themarkersofcholinergicnerves(ChAT)andICCs(c-kit)wereused
7、todouble-immunostainingwithHCN2channel.ResultsMostofHCN2-positiveIRcellsforHCN2withinthemyentericplexusofmaturemicewereDogielIΙtype-neuronsandIRpositiveproductsoccurredonthecellmembranesandcytoplasm.tTheyoftengatheredinclusterintheganglia,whichcontainsedseveraltodozensofIRcell
8、sforHCN2.IRproductscanalsobewereseenonnervousprocess,butmajor
