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《體內(nèi)外人脂肪間充質(zhì)干細(xì)胞向肝樣細(xì)胞分化的實(shí)驗(yàn)研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫(kù)。
1、萬(wàn)方數(shù)據(jù)·322·生堡墅里叢壁苤查；!!!至±旦箜!!鲞箜!塑曼墅!』旦!旦!塑塑堡!￡墅堡：壘2型!!!!!∑!!：!!!墮!：!體內(nèi)外人脂肪間充質(zhì)干細(xì)胞向肝樣細(xì)胞分化的實(shí)驗(yàn)研究殷莉波趙文秀尹震宇楊中萌張瓊王效民【摘要】目的分離、培養(yǎng)入脂肪問(wèn)充質(zhì)干細(xì)胞(hADsCs)，研究其在體內(nèi)外向類肝樣細(xì)胞轉(zhuǎn)化的能力。方法采用I型膠原酶消化皮下脂肪，貼壁分選去除造血細(xì)胞的方法獲得較純的干細(xì)胞。通過(guò)對(duì)形態(tài)觀察、表面分子鑒定、透射電鏡內(nèi)部結(jié)構(gòu)掃描以及成脂、成骨定向誘導(dǎo)對(duì)脂肪間充質(zhì)干細(xì)胞進(jìn)行鑒定。用EGF、FG
2、F、OSM、HGF、TSA細(xì)胞因子體外誘導(dǎo)hADSCs向肝樣細(xì)胞分化。尾靜脈輸注hADSCs至急性肝損傷裸鼠，觀察hADSCs在裸鼠肝臟內(nèi)定植、向肝樣細(xì)胞分化情況，以及hADSCs對(duì)裸鼠肝損傷的保護(hù)作用。結(jié)果分離獲得的細(xì)胞呈長(zhǎng)梭形貼壁生長(zhǎng)，低表達(dá)CD34、CD45，高表達(dá)CD73、CD90、CDl05，具有多向分化潛能。誘導(dǎo)細(xì)胞表現(xiàn)出肝樣細(xì)胞形態(tài)，表達(dá)AFP、alb，并且和肝細(xì)胞一樣具有吸脂性。體內(nèi)誘導(dǎo)：移植細(xì)胞在裸鼠體內(nèi)生存、分布，并且細(xì)胞表達(dá)人alb，細(xì)胞體積較裸鼠肝臟細(xì)胞大，形態(tài)不均一，與
3、人肝臟細(xì)胞形態(tài)有差異。同時(shí)研究發(fā)現(xiàn)hADSCs可改善急性肝損傷裸鼠肝功能，降低轉(zhuǎn)氨酶。結(jié)論人脂肪間充質(zhì)干細(xì)胞可在體內(nèi)外向肝樣細(xì)胞分化，分化的細(xì)胞具有肝細(xì)胞樣生物學(xué)性狀。hADSCs對(duì)裸鼠急性肝損傷具有一定的治療作用?！娟P(guān)鍵詞】脂肪；問(wèn)充質(zhì)干細(xì)胞；分化Experimentalstudyofhumanadiposederivedmesenchymalstemcellsdifferentiatedintohepatocyte-likeeelIsin試VOandvitroYlNLi-bo’．zHA0We
4、n-xiu。YlNZhen-yu，YANGZhong-meng，zHANGQiong，WANGXiao-min．’DepartmentofHepatobiliarySurgery，Af矗liatedXiamenZhongshanHospiral0fXiamenUniversity。Xiamen361004，P．R．ChinaCorrespondingauthor：WANGXiao-min，Email：wxm2203@z優(yōu)“．edu．鯽[Abstract]0bjectiveToisolateand
5、culturehumanadiposederivedmesenchynalstemcells(hADSCs)andstudythepotentialofhADSCStOdifferentiateintohepatocyte-likecells．MethodsToensuretheremovalofcontaminatinghematopoieticcells，hADSCswereselectedbasedonplasticad—herence．CellsuHaceantigenwasconfir
6、medbyflowcytometry；ultramicrostructurewasdetectedbytransmissionelectronmicroscopy；adipogenicandosteogenicdifferentiationofhADSCswasanalyzedbyo訂RedOstainingandyonKossastaining．hADSCswereexposedtOdifferentiationmediumcontai—ningEGF，F(xiàn)GF，0SM．HGF，TSAinvit
7、ro．10％CCh(100p1／20gbodyweight)wasinjectedintoim—mune-deficientBALB／c-numiceandhADSCs(5×105cells)weresimultaneouslyadministratedfromthetailvein．Bloodsampleswerecollectedandconcentrationofaminotransferaseanddirecthilirubinweredetected．Administrationwit
8、houthADSCswasusedasacontr01．Onemonthlater，wesacrificedthemiceandliversectionswereexaminedbyhistochemica“mmunofluorescencewithhumanALBspe-cificantibedies．ResultshADSCsexhibitedfibroblast-likemorphology，andexpressedCD73，CD90，CDl05．a(chǎn)ndwerelackingofCD34a
