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1、HEREDITAS(Beijing)2008年6月,30(6):795―800ISSN0253-9772www.chinagene.cn研究報(bào)告DOI:10.3724/SP.J.1005.2008.00795歐洲黑楊基因資源材性關(guān)聯(lián)基因的SNP分析122丁明明,黃秦軍,蘇曉華1.國(guó)家林業(yè)局國(guó)有林場(chǎng)和林木種苗工作總站,北京100714;2.中國(guó)林業(yè)科學(xué)研究院林業(yè)研究所國(guó)家林業(yè)局林木培育重點(diǎn)實(shí)驗(yàn)室,北京100091摘要:以115個(gè)歐洲黑楊(PopulusnigraL.)無(wú)性系為材料,利用TaqMan技術(shù)分析了歐洲黑楊基因資源參與木質(zhì)素和纖維素合成的酶(4CL、P
2、AL和CesA2)的單核苷酸多態(tài)性,并對(duì)分型的SNPs與木材材性性狀(物理性狀：基本密度、纖維長(zhǎng)、纖維寬、微纖絲角;化學(xué)性狀:木質(zhì)素含量、纖維素含量、α纖維素含量等)進(jìn)行了相關(guān)分析。結(jié)果如下:(1)在對(duì)4CL、PAL和CesA2等3個(gè)基因進(jìn)行檢測(cè)時(shí),共獲得27個(gè)SNPs標(biāo)記,對(duì)其中轉(zhuǎn)換(A-G,C-T)有17個(gè)位點(diǎn),顛換(A-C,G-C,G-T,A-T等)有10個(gè)位點(diǎn);(2)對(duì)其中的3個(gè)SNPs進(jìn)行了分型,分別記作SNP1、SNP2和SNP3;(2)對(duì)已經(jīng)分型SNPs位點(diǎn)與材性性狀進(jìn)行方差分析,結(jié)果顯示,3個(gè)SNPs中只有SNP1與4年生歐洲黑楊綜纖維素含量
3、顯著相關(guān),表現(xiàn)為負(fù)效應(yīng),貢獻(xiàn)率為11.11%;(3)對(duì)歐洲黑楊4CL基因的SNP1標(biāo)記的不同基因型所對(duì)應(yīng)的材性性狀進(jìn)行方差分析,結(jié)果顯示基因型為CC和CT的歐洲黑楊相對(duì)于基因型為TT的歐洲黑楊有較高的纖維素含量。關(guān)鍵詞:歐洲黑楊;材性;單核苷酸多態(tài)性AnalysisonSNPslinkedwithwoodpropertiesofPopulusnigraL.generesources122DINGMing-Ming,HUANGQin-Jun,SUXiao-Hua1.GeneralAdministrationofStateForestFarms,Treeseed
4、sandseedlings,SFA,Beijing100714,China;2.TheResearchInstituteofForestry,CAFLaboratoryofTreeBreedingandCultivation,SFA,Beijing100091,ChinaAbstract:Inthisstudy,115PopulusnigraL.collectedfromEuropewereusedasmaterials.TheyweredetectedforSNPsofgeneslinkedwithligninandholocellulose(4CL,PA
5、LandCesA2)byTaqMantechnologyandcorrelationsbetweenSNPsandwoodproperties(thewoodspecielygravity,fiberlength,fiberwidth,microfibreangle,lignin,holocellulosecontentandα-Cellulosecontent)werealsostudied.resultsshowedthat(1)27SNPswereobtainedinthegenes,suchas4CL,PALandCesA2associatedwit
6、hligninsynthesis.Amongthem,17SNPsweretransition(A-G,C-T),10SNPsweretransversion(A-C,G-C,G-T,andA-T).(2)ThreeoftheSNPswerediscriminated,asignificantnegativecorrelationbetweenholocellulosecontentoffour-yearoldP.nigraandSNP1wasdetected,andcontributionratiowas11.11%.SNP2andSNP3hadnosig
7、-nificantcorrelationwithwoodproperties.(3)WoodpropertiesofvariousgenotypesofSNP1weresignificantlydifferent.CCandCTgenotyperelativetoTTgenotypehadlargerholocellulosecontent.SNP1wouldbeanefficientmarkertochoose收稿日期:2007?11?23;修回日期:2008?01?23基金項(xiàng)目:國(guó)家“十一五”科技支撐計(jì)劃課題(編號(hào):2006BAD01A15)和國(guó)家高技術(shù)
8、研究發(fā)展“863”計(jì)劃課題(編號(hào):2006AA100
