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《細(xì)胞代謝組學(xué)用于羽扇豆醇干預(yù)人乳腺癌細(xì)胞 MCF-7的機(jī)理探究-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、2014年3J]色譜Vo1．32NO．3March20l4ChineseJournalofChromatography278～283研究論文DOI：10．3724／SP．J．1123．20l3．1l010摘要：應(yīng)川丁氣相色譜一質(zhì)譜聯(lián)J1](GC—MS)的代謝組學(xué)方法結(jié)合細(xì)胞用期實(shí)驗(yàn)，研究制扇雕體外抑制人乳腺癌鄉(xiāng)【H胞MCF一7增殖的作用機(jī)理。代謝組學(xué)的研究結(jié)果表明：通過(guò)交偏最小方差判別分析(OPLS—DA)叮以很好地區(qū)分羽痢～Z雕f1：用的MCF一7細(xì)胞代謝譜與對(duì)照紺細(xì)胞代鰣譜，模型參數(shù)為：R=Ycum=0．988，QYcum=0．964VIP(variable
2、importanceintheprojection)值大于1的差異代謝物進(jìn)一步川t檢驗(yàn)進(jìn)干J-化分析，選擇t<0，05(VIP>1)的代f9If物作為羽扇豆醇作用組的生物標(biāo)志物，得到琥珀酸、磷酸、亮氨酸、亮氨酸等l1種代塒差異物。結(jié)合羽崩豆醇將細(xì)胞胤期抑制侄G1期這一現(xiàn)象，推測(cè)羽扇醇町能是主要抑制r三羧酸循環(huán)f}J的琥舶n先輔酶A的乍成和底物磷酸化，ij成ATP的反應(yīng)來(lái)抑制MCF一7細(xì)胞的增殖。本實(shí)驗(yàn)從代謝組學(xué)角度為乳腺螂抗jIf1瘸rdL$提供新的線索關(guān)鍵詞：氣卡l{色譜一質(zhì)譜；羽扇醇；MCF一7細(xì)胞；乳腺癌；抑制機(jī)理；交偏最小方差判別分析；細(xì)胞代謝組學(xué)；細(xì)胞
3、川期中圖分類號(hào)：0658文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1000—8713(2014)03—0278—06Abstract：TheobjectiveofthisresearchistoinvestigatethesuppressiveeffectsoflupeolonMCF一7breastcancercells，andexploreitsmechanismoninhibitingtheproliferationofMCF一7cellsbasedoncellmetabonomicsandcellcycle．Gaschromatography—massspectrometr
4、y(GC—MS)wasusedinthecellmetabonomicsassaytoidentifymetabolitesofMCF·7cellsandMCF一7cellstreatedwithlupeo1．Then，orthogonalpartialleastsquaresdiscriminantanalysis(OPLS—DA)wasusedtoprocessthemetabolicdataandmodelparametersofOPLS—DAwereasfol—lows：RYcum=0．988，QYcum=0．964，whichindicatedthat
5、thesetwogroupscouldbedistin—guishedclearly．Themetabolites(VIP(variableimportanceintheprojection)>1)wereana—lyzedbyt-test，andfinally，metabolites(t<0．05)wereidentifiedtobebiomarkers．Elevenmetabolitessuchasbutanedioicacid，phosphoricacid，L—leucineandisoleucinewhichhadasignificantcontribu
6、tiontoclassificationwereselectedandpreliminarilyidentifiedduetotheaccuratemass．CellcycleassaywasanalyzedbyFACSCalibur．SincethecellsinthephaseofGlwereincreasedsignificantlyafterthetreatmentoflupeol，wespeculatedthatlupeolhasabloc—kingefectonthegenerationofsuccinyl—CoAandthereactionofsu
7、bstratephosphorylatiOnoftricarboxylicacidcycleofMCF一7cells．ThisstudyprovidedanovelapproachtothemechanismresearchonthelupeoltreatmentonMCF一7breastcancercellsbasedoncellmetabonomics．Keywords：gaschromatography—massspectrometry(GC—MS)；lupeol；MCF一7cells；breastcancer；inhibitionmechanism；or
8、thogonalpart
