吡非尼酮及鹽酸氨溴索對(duì)百草枯誘導(dǎo)的大鼠急性肺損傷保護(hù)作用的比較

《吡非尼酮及鹽酸氨溴索對(duì)百草枯誘導(dǎo)的大鼠急性肺損傷保護(hù)作用的比較》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、成都醫(yī)學(xué)院學(xué)報(bào)2014年第9卷第1期JournalofChengduMedicalCollege，2014，Vo1．9，No．1·l5·doi：10．3969／j．issn．16742257．2014．O1．005·論著·吡非尼酮及鹽酸氨溴索對(duì)百草枯誘導(dǎo)的大鼠急性肺損傷保護(hù)作用的比較韓楓，凌心(徐州市腫瘤醫(yī)院藥劑科，徐州221005)【摘要】目的比較吡非尼酮(pirfenidone，PF)及鹽酸氨溴索(ambroxolhydrochloride，AH)對(duì)百草枯(paraquat，PQ)誘導(dǎo)的大鼠

2、急性肺損傷的保護(hù)作用。方法4O只雄性成年SD大鼠隨機(jī)分為正常對(duì)照組、PQ組、PQ+PF組、PQ+AH組，采用腹腔注射2oPQ溶液誘導(dǎo)大鼠急性PQ中毒模型。蘇木精伊紅(HE)染色評(píng)價(jià)肺組織損傷情況；測定肺組織濕重／干重比(W／D)；測定各組大鼠肺組織中超氧化物歧化酶(SOD)活性、丙二醛(MDA)、谷胱甘肽(GSH)含量變化；ElISA法檢測血清中腫瘤壞死因子a(TNF一)及白細(xì)胞介素(II一6)含量。結(jié)果與正常對(duì)照組大鼠相比，PQ組大鼠肺組織肺泡萎縮，部分過度膨脹，組織炎性侵潤和纖維化明顯，肺組

3、織氧化應(yīng)激水平明顯升高，肺組織W／D增加，血漿中TNF_a及II一6含量增加；與PQ組相比，給予PF或AH治療后大鼠肺組織損傷和纖維化降低，大鼠肺組織氧化應(yīng)激水平降低，肺組織W／D降低，血漿中TNFa及II一6含量降低。PF與AH組相比，各指標(biāo)差異無統(tǒng)計(jì)學(xué)意義。結(jié)論P(yáng)F和AH均可以減輕PQ中毒大鼠肺組織損傷，其治療作用可能與其抗炎抗氧化有關(guān)?！娟P(guān)鍵詞】百草枯；急性肺損傷；吡非尼酮；鹽酸氨溴索【中圖分類號(hào)】R595．4【文獻(xiàn)標(biāo)志碼】AProtectiveEffectsofPirfenidonean

4、dAmbroxolHydrochlorideAgainstParaquat_InducedLungDamageinRatsHANFeng，LINGXin(TheDepartmentofPharmacy，XuzhouTumourHospital，Xuzhou221005，China)[Abstract]ObjectiveTostudytheprotectiveeffectsofpirfenidone(PF)andambroxolhydrochloride(AH)againstparaquat(PQ

5、)一inducedlungdamageinratsanditsunderlyingmechanisms．MethodsFourtyratswererandomlydividedintofourgroups：controlgroup，PQgroup，PQ+PFgroup，andPQ+AHgroup．Thepoisoningmodelwasinducedbyintragastricallyadministering20percentPQsolution(25mg／kg)．Theratlungtiss

6、uemorphologicalchangeswereobservedwithHEstaining，theratioofwettodrylungtissue(W／D)wasexamined，theactivityofsuperoxidedismutase(S()D)，thecontentofmalondialdehyde(MDA)andglutathione(GSH)wereexaminedinthelungtissue，thecontentofII6andTNF—dweredeterminedu

7、singenzyme—linkedimmunosorbentassay(EIISA)kits．ResultsComparedwiththecontrolrats，thedegreeoflungdamagewassevere，thelevelsofoxidativestressandtheratioofW／Dwereincreased，andtheIL6andTNF—dintheserumwereincreased．ComparedwiththePQrats，PForAHexhibitedsign

8、ificantdecreaseinoxidativestressandinflammation，theratio0fW／DandtheII6andTNF一Ⅱi11theserumwerealsodecreased．TherewasnodifferencebetweenPFandAHgroups．ConclusionTheresultsindicatethatPFandAHhasaprotectiveeffectagainstPQ-inducedlungdamageinrats．Itisconsi