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1、JournalofChinesePharmaceuticalScienceshttp：／／www．jcps：ae；enChemicalconstituentsfromtheleavesofPremnamicrophyllaTurczZhengxiHu，YongboXue，GuangminYao，ZengweiLuo，YanyanWang，YonghuiZhangJ．HubeiKeyLaboratoryofNaturalMedicinalChemistryandResourceEvaluation，
2、SchoolofPharmacy,TongfiMedicalCollegeHuazhongUniversityofScienceandTechnology,Wuhan430030,China2．FirstCollegeofClinicalMedicalScienceofChina，ThreeGorgesUniversity＆IqchangCentralPeople'sHospital,Yichang443003，ChinaCLCnumber：R284Documentcode：AArticleID：
3、1003-1057(2013)5M31-041．IntrOductiOnThegenusPremna(Verbenaceae)，widelydistributedinHH×火HOHtropicalandsubtropicalareas，suchasAsia，Africa，．I八Australia．a(chǎn)ndthePacificislandsiSallimportantmedicinalHplant[IJThisgenusconsistsofmorethan200speciesin．3theworld，
4、ofwhichabout45speciesaredistilbutedinChina．Sofarseveralditerpcnes，sequitcrpcnes，iridoids，andflavonoidshavebeenisolatedandidentifiedfromthisgenus，andtheywerefoundtoshowawidespectrumofHpharmacologicaleffects，suchasanticancer，antibacterial，OHantifunga1．a(chǎn)
5、ndanti—leishmaniaactivitiesThespecies456P．microphyllaTurcziSlocallyknownas“DOUfuchai”inChina．widelyusedtotreatdysenteryandappendicitisjyo。。Atpresent，therelatedstudiesonthisplantfocusonthe~nutritionandproteinsafetyofits1eaves，whilethere叩pear?Hd．oHH，toh
6、avebeenrarelypreviousstudiesonthebioactivecon—79stituents．Asanefforttodiscoverthenewanticanceragentsfromnatureresource，aphytochemicalinvestigationonthechloroform．solubleextractoftheplant1edtotheisolationof13knowncompounds(Fig．1)，andcompounds2-13areiso
7、latedfromthisgenusforthefirsttime．H2．Experimental2．1．GeneralproceduresNMRspectrawererecordedonaBrukerAM——400Received：2013—0228；Revised：20l3-03—25；Accepted：2013-04-02．HHFoundationitems：NationalNaturalScienceFoundationOfChinarGrantNo．31270390)andProgram
8、forNewCenturyExcellentHTalentsinUniversity(GrantNo．NCET-08—0224)．H2324Correspondingauthor．Te1．：+86-717-6487741：+86—27-836923ll；E—mail：wangyyl001@163．corn；zhangyh@mails．tjmu．edu．cnhttp：／／dx．doi．org／1O．5246@ps．2013．05．063Figure1．Chemicalstructur
