資源描述：
《tim1信號(hào)通路在iga腎病大鼠發(fā)病機(jī)制中的作用》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、-------摘要結(jié)論：1.本分析建立以BSA+CCl4+LPS誘導(dǎo)的大鼠IgAN模型，并發(fā)現(xiàn)TIM-1mRNA的表達(dá)上調(diào)，且與IgAN大鼠的24尿蛋白定量，腎臟病理組織學(xué)損傷程度正相關(guān)，提示TIM-1信號(hào)通路可能參與大鼠IgAN的發(fā)病及進(jìn)展。2.IgAN大鼠腎組織中TIM-1與IL-4表達(dá)均上調(diào)并呈正相關(guān)，提示TIM-1信號(hào)通路可能參與調(diào)控Th2優(yōu)勢(shì)反應(yīng)，而參與IgAN的發(fā)病。3.IgAN大鼠腎組織中TIM-1的表達(dá)與IFN-γ/IL-4表達(dá)呈負(fù)相關(guān)，提示TIM-1信號(hào)通路可能通過(guò)調(diào)控Th1/Th2平
2、衡來(lái)參與IgAN的發(fā)病。關(guān)鍵詞：IgA腎??；TIM-1；IL-4；IFN-γ；FOXP3IV-----------AbstractABSTRACTObjective:TostudytherelationshipbetweenT-cellimmunoglobulindomainandmucindomain-1(TIM-1)andTcellsubsetsinIgAnephropathy(IgAN)ratsandnormalrats,andtoexploremechanismofTIM-1signalingp
3、athwayactinIgANpathogenesis.Method:1.EstablishexperimentalmodelofIgANrat:20SDmaleratswererandomly
dividedintoIgANmodelgroupandnormalcontrolgroup.IgANmodelgroupwere
subcutaneouslygivento0.1%bovineserumalbumin(BSA)intragastriceveryother
day+CCL4mixturedwit
4、hcastoroilperweek+lipopolysaccharide(LPS)tailvein
injectiontwicetoestablisharatmodelofexperimentalIgAN;andnormalcontrol
groupweretreatedwithequalvolumeofPBSandsaline.2.IdentificateIgANratsmodelandobserveindexesduringexperiment:To
observegeneralconditions
5、andotherindicatorsofratsincluding24hurinaryprotein,
serumalbumin,serumcreatinine.ratswerekilledinthe9thweekendandratskidney
werekeptforHEandimmunofluorescence.partsofthekidneytissuesweregained
forreal-timequantitative,andimmunohistochemicalmethodsforTIM-
6、1、IL-4、IFN-γ
andFOXP3expression.3.Comparekidneypathologicallesion,immunohistochemicalexpressionand
TIM-1mRNAexpressionbetweenthetwogroups.AnalysisTIM-1expressionand
clinicalorpathologicaldatainIgANgroup.Result:1.Thegeneralconditionsbetweentwogroups;IgANm
7、odelgrouphadnosignificantdifferencefromcomparisoncontrolgroup
atthebeginning.Lackingofexercise,weightgainslowingdownwerehappendedin
the6thweekend,andgotworseinthe8thweekend.Comparedwithcontrastcontrol
groupweightgain,weightgaininIgANratshadbeensignifican
8、tlysloweddownsince
the6thweekend,thedifferencewasnotstatisticallysignificant(P<0.01).
2.ThekidneypathologyandrenaldamageRatingofrats:V-----------AbstractIgAimmunofluorescenceofnormalcontrolgroupwasnegative,withlightchangei
