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1、-------摘要結(jié)論：1.本分析建立以BSA+CCl4+LPS誘導的大鼠IgAN模型，并發(fā)現(xiàn)TIM-1mRNA的表達上調(diào)，且與IgAN大鼠的24尿蛋白定量，腎臟病理組織學損傷程度正相關，提示TIM-1信號通路可能參與大鼠IgAN的發(fā)病及進展。2.IgAN大鼠腎組織中TIM-1與IL-4表達均上調(diào)并呈正相關，提示TIM-1信號通路可能參與調(diào)控Th2優(yōu)勢反應，而參與IgAN的發(fā)病。3.IgAN大鼠腎組織中TIM-1的表達與IFN-γ/IL-4表達呈負相關，提示TIM-1信號通路可能通過調(diào)控Th1/Th2平
2、衡來參與IgAN的發(fā)病。關鍵詞：IgA腎??；TIM-1；IL-4；IFN-γ；FOXP3IV-----------AbstractABSTRACTObjective:TostudytherelationshipbetweenT-cellimmunoglobulindomainandmucindomain-1(TIM-1)andTcellsubsetsinIgAnephropathy(IgAN)ratsandnormalrats,andtoexploremechanismofTIM-1signalingp
3、athwayactinIgANpathogenesis.Method:1.EstablishexperimentalmodelofIgANrat:20SDmaleratswererandomly
dividedintoIgANmodelgroupandnormalcontrolgroup.IgANmodelgroupwere
subcutaneouslygivento0.1%bovineserumalbumin(BSA)intragastriceveryother
day+CCL4mixturedwit
4、hcastoroilperweek+lipopolysaccharide(LPS)tailvein
injectiontwicetoestablisharatmodelofexperimentalIgAN;andnormalcontrol
groupweretreatedwithequalvolumeofPBSandsaline.2.IdentificateIgANratsmodelandobserveindexesduringexperiment:To
observegeneralconditions
5、andotherindicatorsofratsincluding24hurinaryprotein,
serumalbumin,serumcreatinine.ratswerekilledinthe9thweekendandratskidney
werekeptforHEandimmunofluorescence.partsofthekidneytissuesweregained
forreal-timequantitative,andimmunohistochemicalmethodsforTIM-
6、1、IL-4、IFN-γ
andFOXP3expression.3.Comparekidneypathologicallesion,immunohistochemicalexpressionand
TIM-1mRNAexpressionbetweenthetwogroups.AnalysisTIM-1expressionand
clinicalorpathologicaldatainIgANgroup.Result:1.Thegeneralconditionsbetweentwogroups;IgANm
7、odelgrouphadnosignificantdifferencefromcomparisoncontrolgroup
atthebeginning.Lackingofexercise,weightgainslowingdownwerehappendedin
the6thweekend,andgotworseinthe8thweekend.Comparedwithcontrastcontrol
groupweightgain,weightgaininIgANratshadbeensignifican
8、tlysloweddownsince
the6thweekend,thedifferencewasnotstatisticallysignificant(P<0.01).
2.ThekidneypathologyandrenaldamageRatingofrats:V-----------AbstractIgAimmunofluorescenceofnormalcontrolgroupwasnegative,withlightchangei
