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1、·280·中華肝膽外科雜志2014年4月第2O卷第4期ChinJHepatobiliarySurg，April2014，V01．20，No．4·論著·PIWIL2蛋白在肝門部膽管癌組織和細(xì)胞株中的表達(dá)及臨床意義溫松奇熊小凡楊光耀楊康俞亞紅丁志強(qiáng)游建陳勇軍【摘要】目的研究PIWI12在肝門部膽管癌組織和細(xì)胞株中的表達(dá)及其與臨床病理特征和根治性切除術(shù)預(yù)后的關(guān)系。方法采用免疫組織化學(xué)方法檢測41例肝門部膽管癌和10例正常膽管組織中PIWI12的表達(dá)；蛋白印跡和細(xì)胞免疫熒光技術(shù)檢測人膽管癌細(xì)胞系QBC。。和人正常膽管上皮細(xì)胞HIBEpic株中PIWIL2的表達(dá)。采用Kaplan—Mei
2、er法對膽管癌根治術(shù)后患者進(jìn)行生存期的單因素及多因素分析。結(jié)果PIWIL2在肝門部膽管癌和QBC。。細(xì)胞中的表達(dá)明顯高于對照組織和HIBEpic細(xì)胞(P<0．05)；PIWI12在低、中分化膽管腺癌的表達(dá)明顯高于高分化腺癌(P<0．05)。單因素生存分析顯示根治術(shù)后PIWIL2高表達(dá)的生存期較短(P<0．05)。多因素分析顯示PIWI12的表達(dá)水平是影響膽管癌根治術(shù)預(yù)后的獨(dú)立危險因素(P<0．05)。結(jié)論P(yáng)IWIL2在肝門部膽管癌組織和細(xì)胞系中表達(dá)上調(diào)，其表達(dá)水平與腫瘤分期及分化程度相關(guān)，是影響肝門部膽管癌根治切除預(yù)后的獨(dú)立危險因素?！娟P(guān)鍵詞】PIWI蛋白，PIWIL2；肝門部
3、膽管癌；預(yù)后；危險因素ExpressionandclinicalsignificanceofPIWIL2inhilarcholangiocarcinomatissuesandincelllinesnSongqi，XiongXiaofan，Guangyao，Kang，Yahong，DingZhiqiang，YouJian，ChenYongjun．DepartmentofHepatobiliaryPancreaticandHerniaSurgery，AffiliatedPuaiHospital，TongMedi—calCollege，HuazhongUniversityofScie
4、nceandTechnology，Wuhan430000，ChinaCorrespondingauthor：ChenYongjun，Email：chenyongjun45@126．corn【Abstract】ObjectiveToexploretherelationshipbetweenP1wII2andclinic叩athologicalcharac—teristiesandprognosisafterradicalresectionbydetectingtheexpressionofPIWII2inhilarcholangiocarcino．mstissuesandince
5、lllines．MethodsTheexpressionofPIWI【2wasdetectedbyimmunohistochemistryin41hilarcholangiocarcinomasand10controltissues，Western．blotandimmunocytofluorescentwereusedtoinvestigatethePIWII2expressionincholangiocarcinomacelllinesQBC939andbileductepithelialcelllinesHIBEpic．Univariateandmultivariatea
6、nalysisofsurvivalwasperformedusingtheKaplan—Meiercurveinhi—larcholan~ocarcinomapatientswhohadunderwentradicalresection．ResultTheexpressionofPIWI12inhilarcholangiocareinomatissuesandQBC939cellsweresignificantlyhigherthanthecontroltissuesandHIBEpiccells(P<0．05)．TheexpressionofPIWI12inpoorandmo
7、deratediferentiatedcholangiocarcino—mawassignificantlyhigherthanthewelldifferentiatedgroup(P<0．05)．UnivariateanalysisshowedthathighexpressionofPIWIL2wasassociatedwithshort—survivaltimeafterradicalresection(P<0．05)．Muhi—variateanalysisshowedthatthee
