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1、·280·中華肝膽外科雜志2014年4月第2O卷第4期ChinJHepatobiliarySurg，April2014，V01．20，No．4·論著·PIWIL2蛋白在肝門部膽管癌組織和細胞株中的表達及臨床意義溫松奇熊小凡楊光耀楊康俞亞紅丁志強游建陳勇軍【摘要】目的研究PIWI12在肝門部膽管癌組織和細胞株中的表達及其與臨床病理特征和根治性切除術(shù)預(yù)后的關(guān)系。方法采用免疫組織化學方法檢測41例肝門部膽管癌和10例正常膽管組織中PIWI12的表達；蛋白印跡和細胞免疫熒光技術(shù)檢測人膽管癌細胞系QBC。。和人正常膽管上皮細胞HIBEpic株中PIWIL2的表達。采用Kaplan—Mei
2、er法對膽管癌根治術(shù)后患者進行生存期的單因素及多因素分析。結(jié)果PIWIL2在肝門部膽管癌和QBC。。細胞中的表達明顯高于對照組織和HIBEpic細胞(P<0．05)；PIWI12在低、中分化膽管腺癌的表達明顯高于高分化腺癌(P<0．05)。單因素生存分析顯示根治術(shù)后PIWIL2高表達的生存期較短(P<0．05)。多因素分析顯示PIWI12的表達水平是影響膽管癌根治術(shù)預(yù)后的獨立危險因素(P<0．05)。結(jié)論PIWIL2在肝門部膽管癌組織和細胞系中表達上調(diào)，其表達水平與腫瘤分期及分化程度相關(guān)，是影響肝門部膽管癌根治切除預(yù)后的獨立危險因素?！娟P(guān)鍵詞】PIWI蛋白，PIWIL2；肝門部
3、膽管癌；預(yù)后；危險因素ExpressionandclinicalsignificanceofPIWIL2inhilarcholangiocarcinomatissuesandincelllinesnSongqi，XiongXiaofan，Guangyao，Kang，Yahong，DingZhiqiang，YouJian，ChenYongjun．DepartmentofHepatobiliaryPancreaticandHerniaSurgery，AffiliatedPuaiHospital，TongMedi—calCollege，HuazhongUniversityofScie
4、nceandTechnology，Wuhan430000，ChinaCorrespondingauthor：ChenYongjun，Email：chenyongjun45@126．corn【Abstract】ObjectiveToexploretherelationshipbetweenP1wII2andclinic叩athologicalcharac—teristiesandprognosisafterradicalresectionbydetectingtheexpressionofPIWII2inhilarcholangiocarcino．mstissuesandince
5、lllines．MethodsTheexpressionofPIWI【2wasdetectedbyimmunohistochemistryin41hilarcholangiocarcinomasand10controltissues，Western．blotandimmunocytofluorescentwereusedtoinvestigatethePIWII2expressionincholangiocarcinomacelllinesQBC939andbileductepithelialcelllinesHIBEpic．Univariateandmultivariatea
6、nalysisofsurvivalwasperformedusingtheKaplan—Meiercurveinhi—larcholan~ocarcinomapatientswhohadunderwentradicalresection．ResultTheexpressionofPIWI12inhilarcholangiocareinomatissuesandQBC939cellsweresignificantlyhigherthanthecontroltissuesandHIBEpiccells(P<0．05)．TheexpressionofPIWI12inpoorandmo
7、deratediferentiatedcholangiocarcino—mawassignificantlyhigherthanthewelldifferentiatedgroup(P<0．05)．UnivariateanalysisshowedthathighexpressionofPIWIL2wasassociatedwithshort—survivaltimeafterradicalresection(P<0．05)．Muhi—variateanalysisshowedthatthee
