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1、者外周血淋巴細(xì)胞MDRlmRNA和MRPmRNA的表達(dá)水平增強(qiáng)；免疫組化結(jié)果顯示P．gP，MRP，TOPO-1I在SCLC中均有不同水平表達(dá)，它們的表達(dá)對(duì)SCLC的耐藥起重要作用，因此檢測(cè)這三種耐藥基因有助于判斷化療療效及預(yù)后；在無法獲得SCLC腫瘤組織情況下，通過抽取外周靜脈血檢測(cè)MDRlmRNA和MRPmRNA的表達(dá)來預(yù)測(cè)化療療效是可行的。碩士研究生傅興寧(病理學(xué))指導(dǎo)教師陳樺主任醫(yī)師關(guān)鍵詞：小細(xì)胞肺癌；外周血；耐藥基因；逆轉(zhuǎn)錄聚合酶鏈反應(yīng)法；免疫組織化學(xué)技術(shù)Detectionoftheexpressionofre
2、sistancegenesinsmallcelllungcancerAbstractSmallcelllungcancer(SCLC)accountsfor15％-20％ofalllungcancers，whichoriginsfromtheneuroendocrinefeaturesofendodermcells．Itisahighlymalignanttumor,withthecharacteristicsofrapidgrowthandearlymetastasis．Inadditiontolittleearly
3、SCLC，chemotherapyandradiotherapywereusuallyinvolvedinmedicaltreatments．Inrecentyears，despitegreatprogressesachievedincombinedtreatmentmeasuresincludingchangesofdrugcombination，drugdosesortimeofoperation，clinicaltherapeuticeffectisstillnotsatisfactory．Multidrugre
4、sistance(MDR)isthemainreasonresponsibleforthefailureofchemotherapyandreplaseoftheSCLC．SomestudiessuggestMDRphenomemonintumorcellsiscloselyrelatedwithoverexpressionofMDR1andMRPgenes．However,idealreversaldrugstargettingforMDRisstillunderresearch．objeet：Todetermine
5、thesignificanceofMDR1gene、MRPgeneTOPO一11geneexpressioninperipheralbloodlymphocytes(PBMCs)ofSCLCpatientsbeforeandafterchemotherapy，andexploretheproteinlevelofP—gP、MRP、TOPO—IIinSCLCtumortissues，aswellasstudythecorrelationofMDRl、MRP、andTOPO—IIexpressioninPBMCsandtu
6、mortissues．Methods：RT-PCRmethodandimmunohistochemistrymethodwereusedinthepresentstudy．Results：1．Detectingthepherepheralbood，theexpressionlevelsoftheMDRlmRNAandMRPmRNAinPBMCsinSCLCgroupwerehi曲erthanthatinthecontrolgroup(P<0．05)．TheexpressionlevelsofMDR1mRNAandMRP
7、mRNAafterchemotherapyinSCLCweresignificantlyhigherthanbeforechemotherapy(P<0．05，O．001)．2．TheexpressionlevelsofMDR1mRNAandMRPmRNAinextensivestageweresignificantlyhigherthaninlimitedstageOfSCLCafterthreecyclesofchemotherapyfP<0．05)．Nocorrelationswerefoundbetweenth
8、eexpressionlevelsofMDR1mRNAandMRPmRNAinPBMCsandage，gender，smokinghistory,tumorsize，clinicalstagesandlymphnodemetastasis(P>0．05)．3．Intumortissues．thepositiveratesofP-g
