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1、牛乳鐵蛋白單克隆抗體的制備沈陽張英華東北農業(yè)大學食品學院，哈爾濱150030摘要：以LF(牛乳鐵蛋白)為抗原對BALB/C小鼠進行免疫，利用雜交瘤技術進行融合，制備了3株抗牛LF的雜交瘤細胞，并對其濃度、上清效價、腹水效價、抗體亞類、分泌單克隆抗體穩(wěn)性定、單克隆抗體分子量等進行鑒定。結果表明，抗體分泌穩(wěn)定，腹水效價達到106，間接ELISA、Westernblot結果顯示3株單抗均與FL發(fā)生特異性結合；Ig亞類鑒定均為IgG，且輕鏈均為K鏈。牛乳鐵蛋白；免疫；鑒定；單克隆抗體S767.5;X17A1005-9369(2011)11-0039-06Prepa
2、rationofmonoclonalantibodyagainstbovinelactoferrinSHENGYangZHANGYinghua2010-12-17國家農業(yè)科技成果轉化資金項目（子課題）沈陽（1983-），女，碩士研究生，研究方向為牛乳鐵蛋白檢測試劑盒的研發(fā)。E-mail:shenyangshipin@163.com張英華，副教授，研究方向為食品科學。E-mail:yhzhang2000@126.comoralpropertiesofporcineplasmaproteinhydrolysateasinfluencedAnalyticalBi
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