資源描述：
《牛乳鐵蛋白單克隆抗體的制備.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、牛乳鐵蛋白單克隆抗體的制備沈陽張英華東北農(nóng)業(yè)大學(xué)食品學(xué)院，哈爾濱150030摘要：以LF(牛乳鐵蛋白)為抗原對BALB/C小鼠進(jìn)行免疫，利用雜交瘤技術(shù)進(jìn)行融合，制備了3株抗牛LF的雜交瘤細(xì)胞，并對其濃度、上清效價(jià)、腹水效價(jià)、抗體亞類、分泌單克隆抗體穩(wěn)性定、單克隆抗體分子量等進(jìn)行鑒定。結(jié)果表明，抗體分泌穩(wěn)定，腹水效價(jià)達(dá)到106，間接ELISA、Westernblot結(jié)果顯示3株單抗均與FL發(fā)生特異性結(jié)合；Ig亞類鑒定均為IgG，且輕鏈均為K鏈。牛乳鐵蛋白；免疫；鑒定；單克隆抗體S767.5;X17A1005-9369(2011)11-0039-06Prepa
2、rationofmonoclonalantibodyagainstbovinelactoferrinSHENGYangZHANGYinghua2010-12-17國家農(nóng)業(yè)科技成果轉(zhuǎn)化資金項(xiàng)目（子課題）沈陽（1983-），女，碩士研究生，研究方向?yàn)榕Ｈ殍F蛋白檢測試劑盒的研發(fā)。E-mail:shenyangshipin@163.com張英華，副教授，研究方向?yàn)槭称房茖W(xué)。E-mail:yhzhang2000@126.comoralpropertiesofporcineplasmaproteinhydrolysateasinfluencedAnalyticalBi
3、ochemistry,1996,239:70-76.bythedegreeofhydrolysis[J].FoodChemistry,2009,56:237-@@[24]KongBH,XiongYL.Antioxidantactivityofzeinhydrolysatesin241.aliposomesystemandthepossiblemodeofaction[J].Journalof@@[20]Alder-NissenJ.Enzymichydrolysisoffoodproteins[M].NewYork:AgriculturalandFoodCh
4、emistry,2006,54:6059-6068.ElsevierAppliedScience,1986.@@[25]SiddhurajuP,BeckerK.Theantioxidantandfreeradical@@[21]DeckerEA,HultinHO.Factorsinfluencingcatalysisoflipidscavengingactivitiesofprocessedcowpea(Vignaunguiculata(L.)oxidationbythesolublefractionofmackerelmuscle[J].Journalo
5、fWalp.)seedextracts[J].FoodChemistry,2007,101:10-19.FoodScience,1990,55:947-950,953.@@[26]KawashimaK,ItohH,MiyoshiM,etal.Antioxidantpropertiesof@@[22]SinnhuberRO,YuTC.2-thiobarbituricacidmethodforthebranched-chainaminoacidderivatives[J].ChemicalandPharmeasurementofrancidityinfishe
6、ryproductsⅡThequantitativemaceuticalBulletin,1979,27:1912-1916.determinationofmalonaldehyde[J].FoodTechnology,1958(12):9-@@[27]Pena-RamosEA,XiongYL,ArteagaGE.Fractionationand12.characterizationforantioxidantactivityofhydrolyzedwheyprotein@@[23]BenzieIFF,StrainJJ.Theferricreducinga
7、bilityofplasma[J].JournaloftheScienceofFoodandAgriculture,2004,84(14):1908-1918.(TRAP)asameasureof"antioxidantpower":TheFRAPassay[J].采用間圭OD450.＞1.0,故趨0.0515￡驗(yàn)中先^@@[1]羅獻(xiàn)梅，陳代文，張克英.乳鐵蛋白及其活性膚的營養(yǎng)生理作用及應(yīng)用前景[J].飼料工業(yè),2005,26(2):5-9.@@[2]PiereeAD.Molecularcloningandsequenceanalysisofbovinel
8、actotransten[J].EurJBiochem,1991,
